Authors: Anadaisy Aguirre, Mark Settles, Chiwah Tseung, Fang Bai
Faculty Mentor: Mark Settles, PhD
College: College of Agricultural and Life Sciences
U2-type and U12-type introns are spliced by different spliceosome complexes during pre-mRNA processing. Reduced efficiency of U12-type RNA splicing results in cell differentiation and proliferation defects in both maize endosperm and the human myeloid blood cell lineage. Endosperm tissue culture screening from 29 mutant lines found the rgh414 mutant that proliferates in endosperm tissue culture similar to the U12 RNA splicing mutants, rgh3 and rbm48. The rgh414 locus was mapped to chromosome 10. Genetic fine-mapping will position the mutant locus to a small chromosomal region to identify candidate RNA splicing factors that could encode the rgh414 gene.