Impact of Terrigenous Organic Matter on Aquatic Microbial Communities and Functions

Jade Bittenbender

Authors:   Jade Bittenbender, Connor Tringali, Anson Tam, Dr. Masanori Fujimoto

Faculty Mentor:  Dr. Masanori Fujimoto

College:   College of Agricultural and Life Sciences


Both aquatic and terrestrial environments go hand in hand with the conversion of inorganic carbon to other organic compounds known as the carbon fixation process. Florida is one of the most vegetated yet lake-rich states in the nation. The inland lakes of Florida provide perfect sites for studying carbon transport and are a major component of the global scale carbon budget. Studying how the terrigenous organic matter in Florida lakes has an effect on the biogeochemical processes and the microbial communities in these lakes will give insight for understanding nutrient cycles on a global scale. We conducted a study that aimed to understand the impact of terrigenous organic matter on lake microbial taxa related to the carbon cycle. Water and sediment samples were collected from six lakes at the Ordway Swisher Biological Station (OSBS) during both dry and wet seasons. Three of the lakes contained inflow from nearby Cypress swamps and three of the lakes were clear with no surface inflow. Microbial diversity and community compositions were determined using 16S rRNA gene-based amplicon sequencing. It was found that organic matter from the forest had an effect on the composition and functions of the microbes in the darker lakes.

Poster Pitch

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Connor Tringali
Connor Tringali (@guest_466)
1 year ago

Hey Jade! I didn’t know you were presenting for OSBS, nice work on the new figures!

Jade Bittenbender
Jade Bittenbender (@guest_1016)
Reply to  Connor Tringali
1 year ago

Hi Connor! Thank you so much!! It was fun being able to update the poster since last time!

Emily (@guest_508)
1 year ago

Very cool!
How often were the sites sample/how were the dates decided?

Jade Bittenbender
Jade Bittenbender (@guest_966)
Reply to  Emily
1 year ago

Thank you Emily! So the we chose to sample during the dry season and the wet season in Florida to examine seasonal effects on the diversity of the microbes. We sampled once per season and then analyzed both the water and sediment samples!

Jade (@guest_682)
1 year ago

Hi Connor! Thank you so much!! It was fun being able to update the poster since last time!

Allen Wysocki - Associate Dean CALS
Allen Wysocki - Associate Dean CALS (@guest_1030)
1 year ago


Well done. Great talk. I loved the way you referred to the poster and your exhibits. Sounds like you had a successful research experience.

Doc W

Jade Bittenbender
Jade Bittenbender (@guest_1356)

Thank you so much Dr. Wysocki!! I am so grateful to have done it with Dr. Fujimoto – he is such a great mentor and I learned so much in his lab! Thank you for visiting my poster!

Masa Fujimoto
Masa Fujimoto (@guest_1442)
1 year ago

Great job Jade in summarizing key points in limited time.

Jade Bittenbender
Jade Bittenbender (@guest_1686)
Reply to  Masa Fujimoto
1 year ago

Thank you Masa!!

Katie Dorman-Sims
Katie Dorman-Sims (@guest_2648)
1 year ago

Great overview of your poster. Your graphs and graphics are very compelling. I am sorry you didn’t get to present in person, but you adapted to the online format very well.

Jade Bittenbender
Jade Bittenbender (@guest_3130)
Reply to  Katie Dorman-Sims
1 year ago

Thank you so much Katie! It was such a fun project to work on! The online symposium is definitely different, but it is getting easier for me to use technology now! 😉