NMR Characterization of Antigen A/C3 Binding in Streptococcus mutans
Authors: Albert Brotgandel, Gwladys Riviere, Jeanine Brady, Joanna Long
Faculty Mentor: Joanna Long
College: College of Medicine
Streptococcus mutans has been studied extensively since it was first isolated from dental caries as the primary etiologic agent of human dental caries. WapA and Adhesin P1 are produced by S. mutans in the early stages of biofilm establishment when sucrose is not present. This enables S. mutans to anchor to the dental surface and create a site for biofilm formation that, if left untreated, can progress to becoming a dental carie. In this study we use NMR to characterize the interaction of two derivatives of WapA and Adhesin P1, named Antigen A (AgA) and C3, respectively. By understanding the binding of these two proteins, we are able to understand their potentially synergistic interaction during the formation of S. mutans biofilm. This understanding is applicable to the use of Antigen A and C3 as targets for biofilm inhibition as a way of improving the treatment of dental caries.
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