Changes in Energy Metabolism Caused by Oncolytic Myxoma Virus Infection in Cancer

Ivanna di Lorenzo

Authors:  Ivanna Di Lorenzo, Rohit Mahar, Mukundan Ragavan, Adam Behar, Matthew E Merritt

Faculty Mentor: Matthew E Merritt

College:  College of Medicine

Abstract

The Myxoma virus (MYXV) has been shown to be a promising candidate to fighting several cancers as an oncolytic virus. Investigating changes in the energy metabolism in four cell lines (RK13- rabbit epithelial cells, SFxL- human glioblastoma cells, HUH-7- human hepatocellular carcinoma cells, and A549- human lung carcinoma) will help in understanding how the myxoma virus targets cancer cells specifically and provide a metabolic basis for its oncolytic activity. The focus of the study is the determination of the changes in citric acid cycle turnover caused by an MYXV infection. All cell lines (control, MYXV infected, and MYXV with metabolic inhibitors) were cultured in media containing [U-13C] glucose. The changes in glucose metabolism will be recorded via nuclear magnetic resonance spectroscopy (NMR) and oxygen consumption measurements. In addition, changes in pool sizes of various metabolites upon myxoma infection will also be determined by gas chromatography-mass spectrometry (GC-MS).

Poster Pitch

Click the video below to view the student's poster pitch.

Poster

Click the image to enlarge.
0 0 vote
Presenter Rating
8 Comments
Oldest
Newest Most Voted
Inline Feedbacks
View all comments
Ivanna di Lorenzo
Ivanna di Lorenzo (@guest_428)
1 year ago

Thank you for visiting my poster! If you have any questions or comments, please leave them below!

Clayton Olinger
Clayton Olinger (@guest_2074)
1 year ago

Hey Ivanna,

Great job on the poster and on the video!

Ivanna di Lorenzo
Ivanna di Lorenzo (@guest_2640)
Reply to  Clayton Olinger
1 year ago

Thank you!

Lily Silsby
Lily Silsby (@guest_2248)
1 year ago

Hi Ivanna! Wonderful work on your poster! Do you know what kind of genome Myxoma viruses have and do they cause diseases in humans?

Ivanna di Lorenzo
Ivanna di Lorenzo (@guest_2770)
Reply to  Lily Silsby
1 year ago

So far the genome for the Myxoma virus infects the normal cells of rabbits, but when we expose human cells to the virus it has only been targeting cancerous cells. As far as we know there are no known diseases caused by the Myxoma virus in humans.

Ivanna di Lorenzo
Ivanna di Lorenzo (@guest_3292)
Reply to  Lily Silsby
1 year ago

And thank you so much!

Peggy Borum
Peggy Borum (@guest_2390)
1 year ago

Any ideas about how the addition of beta-hydroxybutyrate or acetoacetate would impact what you are seeing?

Ivanna di Lorenzo
Ivanna di Lorenzo (@guest_3498)
Reply to  Peggy Borum
1 year ago

I talked to my research coordinator, and so far we can’t comment on ketone bodies because we haven’t looked into it!