Authors: Ivanna Di Lorenzo, Rohit Mahar, Mukundan Ragavan, Adam Behar, Matthew E Merritt
Faculty Mentor: Matthew E Merritt
College: College of Medicine
The Myxoma virus (MYXV) has been shown to be a promising candidate to fighting several cancers as an oncolytic virus. Investigating changes in the energy metabolism in four cell lines (RK13- rabbit epithelial cells, SFxL- human glioblastoma cells, HUH-7- human hepatocellular carcinoma cells, and A549- human lung carcinoma) will help in understanding how the myxoma virus targets cancer cells specifically and provide a metabolic basis for its oncolytic activity. The focus of the study is the determination of the changes in citric acid cycle turnover caused by an MYXV infection. All cell lines (control, MYXV infected, and MYXV with metabolic inhibitors) were cultured in media containing [U-13C] glucose. The changes in glucose metabolism will be recorded via nuclear magnetic resonance spectroscopy (NMR) and oxygen consumption measurements. In addition, changes in pool sizes of various metabolites upon myxoma infection will also be determined by gas chromatography-mass spectrometry (GC-MS).