Authors: Ryan Fishman, Alexis Trumbull
Faculty Mentor: Jorg Bungert
College: College of Medicine
TFII-I is a transcription factor known to both positively and negatively impact gene expression in cells in response to stress, cell cycle, and cell differentiation. This study analyzes TFII-I function throughout hematopoiesis using mice in which exon 3 of the TFII-I gene was conditionally deleted via the Cre-Lox Recombinase (LoxP) system. We analyzed gene expression and hematopoietic cell counts in the TFII-I depleted and in control mice. Conditional deletion of TFII-I has been associated with a statistically significant decrease in the number of megakaryocytes and has been seen to have a mild effect on red blood cell production. We observed a 2-fold increase in the number of megakaryocytes (n=3). We also generated a DNA construct for the introduction of a biotinylatable tag into the TFII-I coding region in HUDEP2 cells using CRISPR/Cas9 technology. We will use streptavidin pull-down to analyze proteins that TFII-I interacts with or genomic DNA loci associated with TFII-I. TFII-I has been implicated in the formation of lymphomas and our studies may shed light on the function of TFII-I that may be relevant to its role in cancer.