Evaluation of Transfection Agent Complexation with Ferucarbotran to Enhance T Cell Labeling
Authors: Leyda S. Marrero-Morales, Angelie Rivera-Rodriguez, Nicole Sarna, Duane Mitchell, Carlos Rinaldi
Faculty Mentor: Carlos Rinaldi
College: Herbert Wertheim College of Engineering
Biomedical imaging can be used to track T cells to develop cell-based immunotherapies. Magnetic Particle Imaging (MPI) is a novel technique that allows non-invasive visualization of superparamagnetic iron-oxide (SPIO) tracers. T cell uptake of SPIO is generally poor, so there is a need to optimize labeling. We investigated the effects of ferucarbotran complexation with two transfection agents (TA), poly-L-lysine and protamine sulfate. These cationic molecules interact electrostatically with the SPIO surface, changing the particle charge and potentially increasing SPIO uptake by T cells. Here we characterize SPIO-TA complexes by evaluating magnetic relaxation, hydrodynamic diameter, zeta potential, and MPI performance. We found that SPIO-TA complexes caused particle aggregation in cell media and water. We also show that increasing concentration of TA in water resulted in a more positive zeta potential, while the opposite trend occurred in media for poly-L-lysine. MPI measurements show that increasing TA concentration did not affect MPI signal. T cell viability studies show that protamine sulfate decreases cell viability more than poly-L-lysine. MPI measurements of SPIO-labeled T cells show a signal increase for both SPIO-TA complexes. In the future, we will investigate the effects of the ferucarbotran and poly-L-lysine complex on T cell labeling at different conditions.
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