Comparison of Rumen Fermentation and Microbiome of Rumen Samples Collected via Cannula or Stomach Tube

Nirali Pathak

Authors:  Nirali Pathak, Hao Guan, Kathy G. Arriola, Carlos Nino de Guzman, Ignacio Fernandez, Felipe Amaro, Adeoye Oyebade, Luiz Ferraretto, Diwakar Vyas  

Faculty Mentor:  Dr. Diwakar Vyas

College:  College of Veterinary Medicine 


The objective of this study was to compare fermentation profile, and microbial diversity from rumen samples collected using rumen cannula or stomach tube in lactating dairy cows. Three ruminally cannulated lactating dairy cows were used as rumen fluid donors. Treatments were rumen fluid collected from Stomach tube (ST) or from rumen cannula (C). The pH of rumen contents was measured immediately after collection and rumen samples were analyzed for volatile fatty acid (VFA), ammonia-N (NH3-N) concentration, and metagenome. Data were analyzed using GLIMMIX procedure of SAS. Rumen pH was greater for ST compared to C (6.87 vs 6.25). However, NH3-N (15.2 vs 10.6 mg/dL) and total VFA (121.8 vs 95.5 mM) was greater for C compared to ST. The bacterial community was dominated by the phylum Bacteroidetes, Firmicutes, and Cyanobacteria, regardless of the rumen fluid collection method. However, the abundance of Bacteroidetes was greater while abundance of Firmicutes was lower for ST compared with C. The rumen fluid collection methods had no effects on Chao 1 and Shannon index. In conclusion, molar proportion of individual VFA, Chao1, and Shannon index were similar while rumen pH, NH3-N, and total VFA were affected by the rumen fluid collection method.

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Anon (@guest_166)
1 year ago

Noice job chief

Nirali Pathak
Nirali Pathak (@guest_6380)
Reply to  Anon
1 year ago

Thank you!

Nirali Pathak
Nirali Pathak (@guest_3372)
1 year ago

Hi everyone! I will be answering questions through comments posted to this page or through this Zoom link!

Diwakar Vyas
Diwakar Vyas (@guest_4768)
1 year ago

Excellent presentation, Nirali! Great job!

Nirali Pathak
Nirali Pathak (@guest_6396)
Reply to  Diwakar Vyas
1 year ago

Thank you for all of your support and guidance Dr. Vyas!

Daria Muklewicz
Daria Muklewicz (@guest_5522)
1 year ago

Hey Nirali, I really learned a lot about how the rumen of the cow works through your research. So proud of you and keep up the good work!

Nirali Pathak
Nirali Pathak (@guest_6406)
Reply to  Daria Muklewicz
1 year ago

Thanks Daria!!

Nicole Abruzzo
Nicole Abruzzo (@guest_6590)
1 year ago

Hi Nirali,
Based on your results, do you predict a shift away from cannulation for this type of sampling?

Nirali Pathak
Nirali Pathak (@guest_6996)
Reply to  Nicole Abruzzo
1 year ago

Yes these results support a shift away from rumen cannulation. Although rumen cannulation proved to provide the best data in terms of fermentation and microbiome diversity, the data from esophageal tube sampling can be just as valuable if future researchers account for those differences.

Additionally, the increasing public concern for animal welfare will require researchers to shift away from cannulation.

Allen Wysocki - Associate Dean CALS
Allen Wysocki - Associate Dean CALS (@guest_7528)
1 year ago


I am sorry that I was not able to join you on your Zoom meeting. There were many students and too little time. I enjoyed watching your presentation. You did a great job explaining your research and what you learned as a result of your research. You poster was very well laid out and effectively communicated your research.

Doc W

Nirali Pathak
Nirali Pathak (@guest_7606)

Thank you, Dr. Wysocki! Seeing that you took the time to watch my presentation means a lot to me!