Authors: Youssef Sadek*, Arjun Deven*, Shourya Sangram*, Annika Holmstrom*
Faculty Mentor: Dr. Michael Harris
College: College of Liberal Arts and Sciences
The activity of Ribonucleotide reductase (RNR) is regulated allosterically. This enzyme mediates the formation of deoxyribonucleotides in DNA synthesis by cleaving the 2’ -OH on ribonucleotides. To better characterize this regulation, we will create the plasmids that allow us to massively produce and purify the RNR protein. RNR genes from thermostable Thermatoga Maritima and pathogenic Pseudomonas aeruginosa were inserted into pMCSG7 and pMCSG28 vectors using Ligation-Independent Cloning. Then, the RNR enzyme will be expressed and purified. Finally, the activity and stability of the RNR enzyme will be characterized. Since the RNR is essential for the life cycle, the synthesized plasmids can serve to the studies of cancer and other pathogenic diseases.