Authors: Sierra Short, Savell Robinson, Adam Wong (Overseeing doctor)
Faculty Mentor: Dr. Adam Wong
College: College of Agricultural and Life Sciences
Serratia marcescens is a species of rod-shaped, Gram-negative bacteria that can infect a variety of hosts, including humans. To identify the potential virulence mechanism of Serratia marcescens in a host, we are using the model organism Drosophila melanogaster. We developed a transposon library from a virulent strain of Serratia marcescens, through which it was observed that extracellular proteins and secondary metabolites produced have directly correlated to an increase in virulence. Metabolite expression in mutant strain correlates to a negative impact on virulence when compared to low expression numbers of metabolites in wildtype strain. We expect to observe differences in the metabolite hosts found in our wild type and mutant candidate. Preliminary data shows 20 differentially abundant metabolites between the nonmutant and candidate mutant S. marcescens. Among those 20, the majority of each are polyamides. For future work we will test if supplementing this metabolite has a negative effect on virulence, allowing infected Drosophila melanogaster to survive when metabolite is orally given. Also, we will use PCR to identify the transposon mutation sites
Nice presentation! I’m surprised to hear S. marcescens has such a large host range, wow. I’m curious about the step where you used Bowtie to find the insertion site! I had to work with that in my R class… I know you had to keep it short for your poster/video, but could you go into a little more detail about that?
Well done. You are very advanced using technology. I enjoyed learning about your research and your findings.