Assessing the Role of the Murine Norovirus Minor Capsid Protein VP2 in Pathogenesis

Jonathan Smith

Authors:  Jonathan Smith, Alexa Roth, Emily Helm, Erin Kirsche, Stephanie Karst

Faculty Mentor:  Stephanie Karst

College:  College of Medicine


Human noroviruses are the leading cause of acute viral gastroenteritis outbreaks in children and adults every year. High morbidity rates prompt the need for a better understanding of the underlying mechanisms of norovirus disease induction. Excitingly, our lab has recently shown that wildtype neonatal mice are susceptible to diarrheal disease when infected by the murine norovirus strain MNV-1, providing a clinically relevant small animal model to study norovirus disease induction. We therefore used this model to test the hypothesis that MNV-1 encodes an enterotoxin responsible for causing disease. We specifically tested the role of the norovirus minor capsid protein VP2 as an enterotoxin based on its shared structural characteristics with the known rotavirus enterotoxin NSP4. As the genetically related strain MNV-3 is attenuated in its capacity to cause diarrhea despite replicating to comparable levels to MNV-1 in the gut, reciprocal VP2 chimeric viruses were generated and litters of neonatal mice were infected and observed for diarrhea. Our preliminary results suggest that VP2 is not a virulence determinant.

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Stephanie Karst
Stephanie Karst (@guest_46)
1 year ago

Great job Jonathan!

Jonathan Smith
Jonathan Smith (@guest_2798)
1 year ago

Hey everyone! Since problems are arising with the comment system, attached is a zoom link if you have any questions. However, I will still reply to any comments/questions posted below. Thanks!

Alexa Roth
Alexa Roth (@guest_3020)
1 year ago

Very polished Jonathan! Your talk and poster came out great.

Emilio Roig
Emilio Roig (@guest_3334)
1 year ago

Great job Jonathan and awesome project! Really interesting as I just learned about norovirus in virology 🙂 Keep up the great work!

Jackson Dillard
Jackson Dillard (@guest_3338)
1 year ago

This is very impressive. Awesome job, Jonathan!

Camryn Means
Camryn Means (@guest_5118)
1 year ago

Great presentation Jonathan!

Peyton Warp
Peyton Warp (@guest_7452)
1 year ago

Great presentation! Very impressive.

Michaela R
Michaela R (@guest_7736)
1 year ago

This is really awesome!!