Adam CN Wong, Faculty

Mentor
Adam CN Wong, Faculty
Project Title
Microbiome Discovery Research On Insects
College
College of Agricultural and Life Sciences
Time Commitment
16-20 hours/week ,20+ hours
Method
Depends on lab funding Paid Research Credit
Location of Research
On-Campus
Possible Co-Authorship
Yes

Project Description

We are looking for undergraduate researchers to participate in microbiome research in various model and non-model insect systems. Current project themes include:

Topic 1 – Discovery of microbes that can influence insect behavior
Students will participate in isolating bacteria and fungi from field-collected insects, then test whether these microbes can attract or repel the insects using a cutting-edge video tracking system.

Topic 2 – The role of microbiome in insect xenobiotic detoxification
Students will conduct laboratory evolution experiment by exposing insects to an insecticide at a sublethal doses for generations. Once the insects have evolved resistance, we will experimentally manipulate their microbiome to test if the resistance evolution is sustained by the microbiome.

Topic 3 – Decode microbiome impact on gene regulatory networks using single-cell transcriptomics
We will use single-cell RNAseq to delineate gene expression changes in response to alteration of the microbiome. The students will assist in dissecting insect tissues (e.g. brains) and prepare tissue dissociation to obtain high quality single cells for high throughput sequencing.
 

Syid

Additional Requirements
None

Contact Information

Email Address
adamcnwong@ufl.edu
Phone Number
3522733977
  • adamcnwong@ufl.edu
  • 3522733977
  • We are looking for undergraduate researchers to participate in microbiome research in various model and non-model insect systems. Current project themes include:

    Topic 1 - Discovery of microbes that can influence insect behavior
    Students will participate in isolating bacteria and fungi from field-collected insects, then test whether these microbes can attract or repel the insects using a cutting-edge video tracking system.

    Topic 2 - The role of microbiome in insect xenobiotic detoxification
    Students will conduct laboratory evolution experiment by exposing insects to an insecticide at a sublethal doses for generations. Once the insects have evolved resistance, we will experimentally manipulate their microbiome to test if the resistance evolution is sustained by the microbiome.

    Topic 3 - Decode microbiome impact on gene regulatory networks using single-cell transcriptomics
    We will use single-cell RNAseq to delineate gene expression changes in response to alteration of the microbiome. The students will assist in dissecting insect tissues (e.g. brains) and prepare tissue dissociation to obtain high quality single cells for high throughput sequencing.
     

    Syid

  • None