Staphylococcus aureus is a formidable, opportunistic human pathogen that can cause a wide variety of infections such as chronic wounds, endocarditis, and osteomyelitis. Implicated in its success as a pathogen is its ability to produce a variety of virulence factors and its highly adaptive metabolism. An important contributor to this fluid metabolism is S. aureus nitric oxide synthase (saNOS), which plays a role in aerobic respiratory function and TCA cycle activity, while also conferring resistance to endogenous oxidative stress, antimicrobial peptides, and antibiotics. A second regulator of S. aureus metabolism, the staphylococcal respiratory response regulator (SrrAB) two-component system, has been implicated as a positive regulator of saNOS expression, and is hypothesized to sense both oxidative and nitrosative stress. With previous research suggesting that saNOS and SrrAB have interplay on the metabolic level, this study aims to further investigate the individual impact of both saNOS and SrrAB and their interplay on virulence factor production and host gene expression. It is hoped that this study will provide insight into the mechanisms through which saNOS and SrrAB contribute to virulence, potentially leading to novel therapeutic strategies against this pathogen.