Autophagy is a cellular remodeling process that recycles or eliminates cellular components. This project analyzes how a high energy diet impacts this process in adipose tissue with the onset of obesity. Western blot analysis and other molecular techniques will be used to identify autophagy markers such as LC3, p62, and pULK1 and examine autophagic flux in a high-fat diet (HFD) model. Obesity is a widespread condition across the globe today. Thus, this project will augment our understanding of this condition, and lead to potential therapeutic options for obesity and other metabolic conditions.