Krishna Vekariya


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Krishna Vekariya

Mentor

Founder & Speaker

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Mr. Francis Rozario

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  • Lakshmyya Kesavalu, B.V.Sc., M.Sc., S.C.C.
  • Nutritional Sciences
  • N/A
  • Association between Periodontal bacteria and systemic disease
  • College of Agricultural and Life Sciences Dean's List Fall 2019, Spring 2021, & Fall 2020; National Merit Scholar
  • B.S./D.M.D. UF 7-year Program; University of Florida Pre-Dental American Student Dental Association, Delta Delta Sigma (Pre-Dental Honor Society), Center of Undergraduate Research Board of Students
  • Alachua County Organization for Rural Needs
  • Cultural Dancing, Fitness & Holistic Health
  • Periodontal bacterial dissemination following time-sequential polymicrobial infection
  • Periodontitis is a chronic inflammatory polymicrobial dysbiotic disease caused by several bacteria including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Fusobacterium nucleatum. Our hypothesis is that sequential polymicrobial infection with Sg+Fn+Pg/Td/Tf collectively has a synergistic pathogenicity. Recent studies have shown that PD may play a role in the etiology of several systemic inflammatory diseases such as Alzheimer’s Disease, rheumatoid arthritis, atherosclerotic vascular disease (ASVD), and diabetes mellitus. To induce these diseases, the oral pathogens from gingival tissue must have gained access to the systemic circulation to infect the target organs. Detecting bacterial genomic DNA in systemic tissue can prove pathogenic dissemination in bodily circulation to internal organs.

    Ten-week-old male and female C57BL/6J mice are used for bacterial infection and two groups as sham-infection. Chronic bacterial infection will be done. After 17 weeks of bacterial infection, mice will be euthanized, blood and tissues (heart, lungs, liver, spleen, kidney, brain) collected & stored in RNA. A small aliquot of each tissue will be homogenized. Total DNA will be extracted from the heart, liver, kidney, spleen, and lungs and PCR will be done. The products resulting from PCR will then be separated using gel electrophoresis and the bands will be visualized to determine presence of bacterial genomic DNA.