With mosquitoes collected in the summer of 2018 and identified to species, I will develop and utilize a dissection technique to determine the prevalence of heartworm in competent mosquito vectors. I will develop a dissection technique with infective stage mosquitoes purchased from a lab. Next, I will identify individuals positive for heartworm including potential new vector species with qPCR. Species of interest will include those identified as competent vectors in our previous paper that has be completed and submitted for publishing.