Batrachochytrium dendrobatidis (Bd) is the chytrid fungus that causes chytridiomycosis, and the disease has a global prevalence that has been recorded in every amphibian-inhabited continent. Real time quantitative PCR is used to detect the disease in a sample. To do so, primers are used that target a specific binding site in the DNA of the Bd fungus. I want to explore the accuracy of current primers in their ability to detect Bd in African frog species, and if any mutations within the fungal DNA have caused a decrease in the confidence of the qPCR primers. The study will be limited to samples already collected from central Africa, including frogs from both Cameroon and Gabon.