Kiran Mital

Kiran Mital

Optimization of Parameters for Aerosolized Nanoparticle Delivery


Kiran Mital


Dr. Chang-Yu Wu


Herbert Wertheim College of Engineering


Increase in anthropogenic air pollutants and resulting respiratory effects demands the need for assessing the toxicity of airborne particles. Dosimetric Aerosol in Vitro Inhalation Device (DAVID) mimics the inhalation of aerosols by collecting and gently impacting them in a collection chamber after condensational particle growth (CPG). This study aims to optimize the parameters for efficient collection and in vitro toxicity assessment. CuO NPs at concentrations of 1, 2.5, 5, and 10 mg/mL were suspended in ethanol and nebulized at 5 lpm with a 6-jet Collison nebulizer for 5 min. The generated aerosols are diluted with compressed air (15 lpm) and transported to DAVID, where particle growth occurs. The particles are collected on a Petri dish. The size distribution of the aerosols was measured prior to entering DAVID with a Scanning Mobility Particle Sizer (SMPS) and at the outlet of DAVID with an Aerodynamic Particle Sizer (APS). A balance in the volumetric flow rates throughout the experimental setup was achieved with rotameters to control the flow. To eliminate any aggregates, an impactor was fixed at the inlet of SMPS. With these controls, CuONPs were shown to have grown from a range of 20-40 nm to 1.2-1.6 μm. Upon increasing the concentrations of CuONPs, the theoretical mass dose increased linearly. The values were 18.18, 185.71, 308.82, and 531.31 µg/cm for 1, 2.5, 5 and 10 mg/mL of CuONPs respectively. This suggests efficient condensational growth, collection, and deposition of CuONPs under the set conditions, that could be explored further for toxicity assessment.


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