Madison Francis

Madison Francis

Novel Bio-ELISA Reliably Detects Human and Murine Tyrosine Hydroxylase


Madison Francis, Martin Badov, Adithya Gopinath, Gerry Shaw, Habibeh Khoshbouei


PhD, PharmD, Habibeh Khoshbouei


College of Medicine


Changes in tyrosine hydroxylase (TH) protein, the rate limiting enzymatic step in synthesis of catecholamines norepinephrine and dopamine, has been implicated in many neurological and neuropsychiatric diseases. Traditionally, TH is detected via methods such as Western blot, immunohistochemistry and immunofluorescence. These methodologies use antibodies generated against TH and provide semiquantitative or qualitative assessments of TH expression. There is an unmet need to develop sensitive assays for the quantitative measurement of TH level, for use in preclinical research and potentially for clinical-translational science. Here, we have developed a highly sensitive and reproducible enzyme linked immunosorbent assay using biotinylated antibodies (Bio-ELISA) capable of detecting TH protein as low as 15pg/mL of TH protein, about a thousand-fold below the detection threshold of 15ng/mL using infrared Western blot imaging.


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