Manjula Somanchi

Manjula Somanchi

Models of Gliomas

Authors

Manjula Somanchi, Ryan Wilson, Bayli DiVita Dean, Connor Francis, Laura Falceto Font, Catherine Flores

Mentor

Dr. Catherine Flores

College

College of Medicine

Abstract

Glioblastoma is a common and primary aggressive brain tumor found in adults. The current treatment protocols consist of surgery, radiation, and chemotherapy. The median prognosis of survival for GBM patients is extremely poor at only 15 months. Current research relies on tumor models to test innovative therapies to treat GBM patients. Such models include the murine glioma cell line KR158Bluc, and primary patient-derived glioma cell lines, such as L-0. KR158Bluc is derived from a C57BL/6 mouse and is an adherent glioma cell line. L-0 is in the form of non-adherent cells as neuro-spheres. These cell lines can be passaged to continue to grow for extended periods. The use of KR158Bluc (in vitro) allows for the completion of killing assays and Drug screening assays to evaluate potential therapies. In addition, some immunotherapies can be evaluated by co-culturing immune cells with target tumor cells in vivo. Since KR158Bluc is derived from a C57BL/6 mouse, we can inject the cell line and evaluate survival after treatment with immunotherapy or other therapeutic strategies. For human-derived lines such as L0, cells can be injected into immunocompromised mice to form tumors. This approach, known as humanized mice, allows us to evaluate therapeutic responses in the context of a human tumor and immune system. Both KR158Bluc and the L-0 cell line provide reliable modes of tumor modeling. Testing on the cell lines that resemble the human GBM and human immune system allows for practical clinical translations and further advancements in innovative therapies.

Poster

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Research Pitch

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